Fig. 3

CYPA depletion prevents the migration and invasion of SOC cells in vitro. a Knockdown of CYPA led to the lower migration degree in CAOV3 and OVCAR3 cells. Photographs showing that at the 0, 20th hour time point migrated cells were present. b Transwell assays using both CAOV3 and OVCAR3 human SOC cells transfected with control or CYPA shRNA plasmids, and the degree of invasion was quantified by the invasion cell number. c Immunoblot assays showed the expression of MMP3 in control or CYPA shRNA-transfected CAOV3 and OVCAR3 cells, respectively. d Immunoblot assays revealed the expression level of MMP9 in control or CYPA depletion CAOV3 and OVCAR3 cells. Results are presented as mean ± SD, * P < 0.05. We have done the Image splicing in Fig. 3