Fig. 5

Knocking down TRPC3 expression blocked E2-associated effects on calcium influx. The effect of E2 on calcium influx is shown in (a, SKOV3 cells) and (c, HEY cells). ATP treatment was used as a positive control. A representative image of intracellular calcium influx ([Ca2⁺]_I) is shown. The EOC cell line SKOV3 was used to analyze the effect of downregulating TRPC3 expression on [Ca2⁺]_I (b, SKOV3 cells) and (d, HEY cells). SiTRPC3 and Sicon were transfected into SKOV3 and HEY cells, which were then incubated on glass coverslips for 48 h. Then, 1 μM E2 was added to the medium at the start of detecting intracellular Ca2 + ., and then stained with the fluorescent dye Fluo-2 AM before observation. [Ca2⁺]_i was determined using the F340/380 ratio. The experiment was repeated three times