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Fig. 3 | Journal of Ovarian Research

Fig. 3

From: RETRACTED ARTICLE: LncRNA CTBP1-AS2 sponges miR-216a to upregulate PTEN and suppress endometrial cancer cell invasion and migration

Fig. 3

CTBP1-AS2 overexpression resulted in upregulation of PTEN. HEC-1 cells were transfected with CTBP1-AS2 expression vector or miR-216a mimic to analyze the interactions between CTBP1-AS2 and miR-216a. Overexpression of CTBP1-AS2 and miR-216a was confirmed at 48 h post-transfection (a). The effects of CTBP1-AS2 and miR-216a overexpression on expression of each other were analyzed by qPCR (b). QPCR and western blot were performed to analyze the effects of CTBP1-AS2 and miR-216a overexpression on the expression of PTEN at mRNA (c) and protein (d) levels, respectively. Mean values of 3 biological replicates were calculated and compared by unpaired t test.*,p < 0.05

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