Fig. 3

Ovarian endometriosis environment sustains neutrophil survival, and ovarian endometriosis cultural supernatants conditioned neutrophils suppress T cell immunity. a Dot plots and statistics analysis of annexin V^—viable non-apoptotic neutrophils exposed to 30 and 60% ovarian endometriosis cultural supernatants (OECS) from ovarian endometriosis patients for 16 h. b Human PBMC were labeled with CFSE and proliferation was induced by anti-CD3/anti-CD28 mAb (1 μg/mL each) in the presence of IL2 (100 U/mL). The degree of proliferation was assessed by analyzing the reduction of CFSE-label after cell division in CD8⁺ lymphocytes by flow cytometry. One representative out of four experiments is shown in the left. Statistical analysis of thepercentage of CD8+ T cell proliferation as shown in the right. (n = 4, * P < 0.05). c Human PBMC were stimulated for 24 h by anti-CD3/anti-CD28 mAb (1 μg/mL each) and 1 × protein transport inhibitor cocktail (Invitrogen) added for the last 6 h. Cells were then stained with CD8 mAb, and intracellular IFN-γ production in CD8⁺ T cells was analyzed by flow cytometry. Statistical analysis of the percentage of IFN-γ⁺ of CD8⁺ T cells was shown