Fig. 1

HGSOC spheroids have increased phosphorylated AMPK and LC3-II as compared with adherent cells. a iOvCa147-MA cells were trypsinized, seeded into ULA plates, and protein lysates were isolated at each time point as indicated. Adherent cell controls (adh) were cultured using standard tissue culture-treated plates for 72 h prior to protein isolation. Immunoblot analysis was performed for p-AMPK (T172), AMPK, and LC3B; tubulin served as a loading control. b Densitometric analysis of p-AMPK/AMPK and LC3-II:I ratio from the immunoblots were tested by one-way ANOVA followed by Dunnett’s multiple comparison test (n = 3) (*, p < 0.05). c OVCAR8 cells were trypsinized, seeded into ULA plates, and protein lysates were isolated at each time point as indicated. Adherent cell controls (adh) were cultured using standard tissue culture-treated plates for 72 h prior to protein isolation. Immunoblot analysis was performed for p-AMPK (T172), AMPK, and LC3B; tubulin served as a loading control. d Densitometric analysis of p-AMPK/AMPK and LC3-II:I ratio from the immunoblots were tested by one-way ANOVA followed by Dunnett’s multiple comparison test (n = 3) (*, p < 0.05)