Fig. 4

AKT signaling pathway is regulated by SAA. a. OVCAR-3 cell was treated with SAA 0 μg/ml and 10 μg/ml 12 h respectively, with representative graphs of OVCAR-3 cells in migration. The data were shown as the means±SD (n = 3). The fold change is 2.46, P = 0.0345, t-test. b. The mRNA of MMP-1, MMP-9, and MMP-12 were assayed after OVCAR-3 cell had been treated with SAA 0 μg/ml and 10 μg/ml 24 h respectively. The data were shown as the means±SD (n = 3). The fold changes respectively are 2.3,1.7,2.0. P = 0.0001,0.0137,0.0224. (c, d) RT-qPCR and Western blot analysis of EMT markers in OVCAR-3 cell treated with SAA 0 μg/ml and 10 μg/ml 24 h respectively. The data were shown as the means±SD (n = 3). The fold changes respectively are 0.35,2.1,3.5,1.6. P = 0.0008,0.0362,0.0013. (e, f). Western blot analysis of phosphorylation AKT in OVCAR-3 cell treated with SAA 0 μg/ml and 10 μg/ml 24 h. The fold changes respectively are 2.18,1.97,0.01. P = 0.0004,0.0006,0.2148. Statistical significance was calculated used t-test(A) and Wilcoxon Signed Rank test with one unpaired t-test per row(B-F). (* P < 0.05, *** P < 0.001, **** P < 0.0001)