Fig. 2
From: WISP2 promotes cell proliferation via targeting ERK and YAP in ovarian cancer cells
WISP2 deletion represses cell proliferation and migration. a WISP2 deletion inhibits ovarian cancer cell growth. ES-2 and HO8910 WT and WISP2 deletion cells (1 × 105) were plated in 6-well culture dishes, and viable cells were counted following trypan blue staining. b WISP2 deletion inhibits anchorage-independent growth of ES-2 and HO8910 cells. Cells were cultured in Matrigel. Colonies that formed on days 12 and 17 are shown. Colony numbers were counted on days 14 to 21. c Wound-healing assay for the migration capability of ES-2 WT and WISP2 deletion cells. d Transwell test to detect the migration capability of ES-2 and HO8910 WT and WISP2 deletion cells. Cells were added to transwells and allowed to migrate for 12 h. Cells at the upper surface of the membrane were removed with cotton swabs, and cells on the bottom surface were stained with hematoxylin and eosin. The experiment was replicated thrice. Error bars represent the standard deviation (s.d.). *p < 0.05, **p < 0.01, ***p < 0.001