Fig. 4

Identification of CXCL1 as a target of miR-27b-5p. A. miR-27b-5p and its wild-type (wt) binding sites in the 3′-UTR of CXCL1. The mutant binding sites (mut) were produced in the complementary site for the seed region of miR-27b-5p. B. psiCHECK-2 carrying CXCL1–3′-UTR wt or psiCHECK-2 carrying CXCL1–3′-UTR mut, along with miR-27b-5p mimics or miR-NC, were cotransfected into SKOV3 and OVCAR3 cells. The luciferase activity was detected using a luciferase reporter assay system. ^**P < 0.05 vs. miR-NC. C. The mRNA levels of CXCL1 in miR-27b-5p overexpressing SKOV3 and OVCAR3 cells were examined using qRT-PCR. D. The protein levels of CXCL1 in miR-27b-5p overexpressing SKOV3 and OVCAR3 cells were examined using western blot analysis. E. The level of CXCL1 in ovarian cancer tissues and adjacent tissues were assessed using qRT-PCR. ^**P < 0.01 vs. adjacent tissues. F. Spearman’s correlation analysis was utilized to examine the expression correlation between miR-27b-5p and CXCL1 mRNA in ovarian cancer tissues