Fig. 4

GAS5 positively regulated IL-6 in KGN. KGN cells were transfected with GAS5 expression vector or GAS5 siRNA to analyze the relationship between GAS5 and IL-6. Transfections were confirmed by qPCR at 24 h post-transfection (a). The effects of overexpression and silencing of GAS5 on the expression of IL-6 in KGN cells were analyzed by qPCR and western blot at mRNA (b) and protein (c) level, respectively. Experiments were repeated 3 times and mean values were presented. *, p < 0.05