Fig. 5

GAS5 and testosterone negatively regulated KGN cell apoptosis and testosterone upregulated the levels of GAS5. The effects of overexpression (a) and silencing (b) of GAS5 on the apoptosis of KGN cells were analyzed by cell apoptosis assay. KNG were treated with 0.1 nM or 10 nM testosterone for 24 h, followed by detection of GAS5 expression(c). Cell apoptosis analysis after KNG were treated with 10 nM testosterone (d). Experiments were repeated 3 times and mean values were presented. *, p < 0.05