Fig. 1

The gene and protein levels of GDF9 and BMP15 in GCs were decreased with increasing age. a The relatively mRNA levels of GDF9 and BMP15 were significantly different between the three groups (P < 0.05). They were decreased with increasing age. GAPDH, β-actin and HPRT were used as the internal controls. b The relatively protein levels of GDF9 and BMP15 were significantly different between the three groups (P < 0.05). They were decreased with increasing age. GAPDH was used as internal control. c A single band at 51 kDa represented GDF9, and 45 kDa represented BMP15 by western blot, respectively