Fig. 6

The effect of miR-140 on insulin-treated granulosa cells reversed by the RAP2A inhibitor. Insulin-treated granulosa cells were co-transfected with shRNA-RAP2A/NC and miR-140. a qPCR and b WB analyses were carried out to evaluate the RAP2A, AKT, and phosphorylated AKT levels in each group. c Quantification of phosphorylated AKT. d MTT staining was used for determining the proliferation of insulin-treated granulosa cells with different transfections. e Flow cytometric analysis showing the extent of apoptosis in the differently treated chondrocytes. f Histogram shows the apoptosis level in granulosa cells with different treatments. N = 3. *P < 0.05, **P < 0.01, ***P < 0.001 vs. indicated group