Fig. 4

a: The effect of progesterone on DCN secretion. MGCs were aspirated from preovulatory follicles (> 17 mm) during IVF and cultured for 4 days with a daily medium exchange. 300 nM of progesterone was added to the media for 24 h. Media was collected and subjected to EIA kit measuring DCN concentration. Concentrations are in absolute values. The results are expressed as mean with ± SEM of 4 independent experiments. * represents significant result (P- 0.02). b-c: Expression levels of decorin in CGCs and MGCs obtain from follicles of endometriosis patients. CGCs and MGCs were aspirated from preovulatory follicles (> 17 mm) of endometriosis patients and from control patients. Cells of 3–4 patients were grouped, total mRNA was extracted and subjected to qPCR with decorin and b-actin primers. The results are expressed as fold change mean ± SEM of three independent experiments. Endometriosis sample was set to 1. (P decorin = 0.008). * marks significant result