Fig. 5

Autophagy decreases cell motility by inhibiting RhoA in ovarian cancer cells. a SKOV3 cells treated with hArgI, rapamycin or hArgI + chloroquine or left untreated. SKOV3 cell extracts were incubated with GST-RBD beads before blotting against RhoA. Total cell lysates which were not incubated with the beads were also blotted against RhoA and used as loading control. Right panel: Bar graph illustrating the amount of RhoA activation expressed as fold change of the activated RhoA normalized to the amount of total protein. The densitometry quantification of active and total RhoA bands was performed in ImageJ. b Caov-3 cells treated with hArgI, rapamycin or hArgI + chloroquine or left untreated. Caov-3 cell extracts were incubated with GST-RBD beads before blotting against RhoA. Total cell lysates which were not incubated with the beads were also blotted against RhoA and used as loading control. Right panel: Bar graph illustrating the amount of RhoA activation expressed as fold change of the activated RhoA normalized to the amount of total protein. The densitometry quantification of active and total RhoA bands was performed in ImageJ. c SKOV3 cells were untreated or treated as indicated with Rapamycin, Rapamycin+RhoA-CA, Luciferase siRNA+hArgI+Chloroquine or RhoA siNRA + hArgI+Chloroquine before being imaged moving randomly in media. The bar graph illustrates the fold change of treated SKOV3 cells migration relative to the control