Table 5 Primary antibodies
Molecule | Type | Antibody Clone/Code | Manufacturer | Dilution | Localization | Control tissue | Antigen retrieval | Interpretation |
|---|---|---|---|---|---|---|---|---|
CK7 | Monoclonal (Mouse) | OV-TL12/30 | Dako | x50 | Cytoplasm | Colorectal carcinoma | Citrate | More than 10 % positive cell with staining intensity score 1 to 3 was defined as positive. |
CK20 | Monoclonal (Mouse) | Ks20.8 | Dako | x50 | Cytoplasm | Colorectal carcinoma | Citrate | More than 10 % positive cell with staining intensity score 1 to 3 was defined as positive. |
CDX2 | Monoclonal (Rabbit) | CDX2-88 | Novocastra | x100 | Nucleus | Colon | Citrate | More than 10 % positive cell with staining intensity score 1 to 3 was defined as positive. |
HER-2 | Monoclonal (Rabbit) | 4B5 | Ventana medical system | Ready to use | Membrane | Mammary gland | Citrate | More than 10 % positive cell with staining intensity score 1 to 3 was defined as positive. |
CK 5/6 | Monoclonal (Mouse) | D5/13 B4 | Dako | x100 | Cytoplasm | Mammary gland | EDTA | More than 10 % positive cell with staining intensity score 1 to 3 was defined as positive. |
Androgen receptor | Monoclonal (Mouse) | AR441 | Dako | x50 | Nucleus | Mammary gland | Citrate | More than 10 % positive cell with staining intensity score 1 to 3 was defined as positive. |
ALDH1 | Monoclonal (Mouse) | clone 44 | BD Biosciences | x500 | Cytoplasm | Kidney | Citrate | More than 10 % positive cell with staining intensity score 1 to 3 was defined as positive. |
CD24 | Monoclonal (Mouse) | SN3 | ThermoFisher | x25 | Membrane | Tonsil | Citrate | More than 10 % positive cell with staining intensity score 1 to 3 was defined as positive. |
CD133 | Monoclonal (Mouse) | AC133/1 | Miltenyi Biotec | x500 | Cytoplasm | Colorectal carcinoma | EDTA | More than 10 % positive cell with staining intensity score 1 to 3 was defined as positive. |
PD-1 | Monoclonal (Mouse) | NAT105 | Abcam | x50 | lymphocytes | Tonsil | Citrate | Even minimally positive lymphocyte with staining intensity score 1 to 3 was defined as positive. |
PD-L1 | Monoclonal (Rabbit) | EPR19759 | Abcam | x250 | Membrane and endomembrane | Placenta | Citrate | More than 10 % positive cell with staining intensity score 1 to 3 was defined as positive. |
ZEB1 | Polyclonal (Rabbit) | ab87280 | Abcam | x100 | Nucleus | MCF, MDA-MB-231 | EDTA | More than 1 % positive cell with staining intensity score 1 to 3 was defined as positive. |
c-Met | Monoclonal (Rabbit) | D1C2 | Cell Signaling Technology | x300 | Membrane and cytoplasm | Colorectal carcinoma | Citrate | More than 10 % positive cell with staining intensity score 1 to 3 was defined as positive. |
EGFR | Monoclonal (Rabbit) | D38B1 | Cell Signaling Technology | x100 | Membrane | Epidermis | EDTA | More than 10 % positive cell with staining intensity score 2 or 3 was defined as positive. |
Snail-2 | Polyclonal (Rabbit) | PAB1923 | Abnova | x200 | Nucleus | MCF, MDA-MB-231 | Citrate | More than 10 % positive cell with staining intensity score 1 or 3 was defined as positive. |