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Table 5 Primary antibodies

From: Survival and biomarker analysis for ovarian mucinous carcinoma according to invasive patterns: retrospective analysis and review literature

Molecule Type Antibody Clone/Code Manufacturer Dilution Localization Control tissue Antigen retrieval Interpretation
CK7 Monoclonal (Mouse) OV-TL12/30 Dako x50 Cytoplasm Colorectal carcinoma Citrate More than 10 % positive cell with staining intensity score 1 to 3 was defined as positive.
CK20 Monoclonal (Mouse) Ks20.8 Dako x50 Cytoplasm Colorectal carcinoma Citrate More than 10 % positive cell with staining intensity score 1 to 3 was defined as positive.
CDX2 Monoclonal (Rabbit) CDX2-88 Novocastra x100 Nucleus Colon Citrate More than 10 % positive cell with staining intensity score 1 to 3 was defined as positive.
HER-2 Monoclonal (Rabbit) 4B5 Ventana medical system Ready to use Membrane Mammary gland Citrate More than 10 % positive cell with staining intensity score 1 to 3 was defined as positive.
CK 5/6 Monoclonal (Mouse) D5/13 B4 Dako x100 Cytoplasm Mammary gland EDTA More than 10 % positive cell with staining intensity score 1 to 3 was defined as positive.
Androgen receptor Monoclonal (Mouse) AR441 Dako x50 Nucleus Mammary gland Citrate More than 10 % positive cell with staining intensity score 1 to 3 was defined as positive.
ALDH1 Monoclonal (Mouse) clone 44 BD Biosciences x500 Cytoplasm Kidney Citrate More than 10 % positive cell with staining intensity score 1 to 3 was defined as positive.
CD24 Monoclonal (Mouse) SN3 ThermoFisher x25 Membrane Tonsil Citrate More than 10 % positive cell with staining intensity score 1 to 3 was defined as positive.
CD133 Monoclonal (Mouse) AC133/1 Miltenyi Biotec x500 Cytoplasm Colorectal carcinoma EDTA More than 10 % positive cell with staining intensity score 1 to 3 was defined as positive.
PD-1 Monoclonal (Mouse) NAT105 Abcam x50 lymphocytes Tonsil Citrate Even minimally positive lymphocyte with staining intensity score 1 to 3 was defined as positive.
PD-L1 Monoclonal (Rabbit) EPR19759 Abcam x250 Membrane and endomembrane Placenta Citrate More than 10 % positive cell with staining intensity score 1 to 3 was defined as positive.
ZEB1 Polyclonal (Rabbit) ab87280 Abcam x100 Nucleus MCF, MDA-MB-231 EDTA More than 1 % positive cell with staining intensity score 1 to 3 was defined as positive.
c-Met Monoclonal (Rabbit) D1C2 Cell Signaling Technology x300 Membrane and cytoplasm Colorectal carcinoma Citrate More than 10 % positive cell with staining intensity score 1 to 3 was defined as positive.
EGFR Monoclonal (Rabbit) D38B1 Cell Signaling Technology x100 Membrane Epidermis EDTA More than 10 % positive cell with staining intensity score 2 or 3 was defined as positive.
Snail-2 Polyclonal (Rabbit) PAB1923 Abnova x200 Nucleus MCF, MDA-MB-231 Citrate More than 10 % positive cell with staining intensity score 1 or 3 was defined as positive.
  1. Abbreviation: CK7 Cytokeratin 7, CK20 Cytokeratin 20, CDX2 Caudal type homeobox 2, HER-2 Human epidermal growth factor receptor 2, CK5/6 Cytokeratin 5/6, ALDH1 Aldehyde dehydrogenase 1, CD24 Cluster of differentiation 24, CD133 Cluster of differentiation 133, PD-1 Programmed cell death 1, PD-L1 Programmed cell death 1- ligand 1, ZEB1 Zinc finger E-box binding homeobox 1, EGFR Epithelial growth factor receptor
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