Fig. 4

Pretreatment with LCK inhibitor followed by LCKi-cisplatin treatment attenuates tumor burden, and treated tumors indicate decreased P-LCK. NSG mice were injected with CP70-luciferase transfected cells followed by pretreatment with LCKi (6 mice) or vehicle (24 mice) for 14 days. LCKi mice were then co-treated with LCKi and cisplatin, and vehicle mice were randomized to further treatment with vehicle, cisplatin, saracatinib, or combination (6 mice per arm) (a). IVIS imaging was obtained on a weekly basis to assess tumor growth (b). IVIS luminescence was corrected to baseline for each arm and assessed over time (c) and at the experimental endpoint (d). Tumors from NSG mice were extracted, and protein lysates were prepared and immunoblotted for protein expression of P-LCK (Y394) and T-LCK. Fold changes of protein expression are shown in the figure, with values normalized to vehicle control. GAPDH was used as a loading control (e). Two tumors per condition were probed, with at least three technical replicates performed. Mouse body weight at day 7, 21, and 28 were obtained for each arm, with weights shown as fold change from baseline (f)