Fig. 4

PAXIP1-AS1 regulated PCBP2 expression via miR-6744-5p. a Potential targets of miR-6744-5p combined from results of miRTarBase, TargetScan and miRDB online tools. b Effect of miR-6744-5p mimics on the expressions of PCBP2, YBX1, EPHA4, FAM102A and NMNAT2. c The binding sequence between PCBP2 and miR-6744-5p. d Luciferase activity of PCBP2-WT/Mut reporter under the transfection of miR-6744-5p mimics. e The RNA pulldown assay was performed to assess the interaction between miR-6744-5p and PCBP2. f Enrichments of PAXIP1-AS1, miR-6744-5p and PCBP2 in the beads conjugated with anti-Ago2 or anti-IgG was tested by the RIP assay. g MiR-6744-5p expression in cells with transfection of miR-6744-5p inhibitor. h PCBP2 mRNA and protein levels were tested in each group by RT-qPCR and western blot. *p < 0.05