Table 1 Molecules tested in ovarian cancer models with MMP-14 and MMP-2
From: The role of MMP-14 in ovarian cancer: a systematic review
First author | Year | Model | Molecule(s) involved | Technique | Result |
|---|---|---|---|---|---|
Kikkawa F [67] | 2005 | SKOV-3 cell line | Dipepeptidyl peptidase IV (DPPIV) | Immunoblotting | DPPIV decreases invasion, increases TIMP and E-cadherin |
Barbolina M [38] | 2006 | DOV13 cell line | Epidermal Growth Factor Receptor (EGFR) | Q-PCR Zymography Three-dimensional collagen invasion assay | MMP-14 activation by collagen type I was investigated as measured by MMP-2 activation in zymography. This activation was induced only by EGR1 but not by EGR-2–4 |
Lin S-W [68] | 2007 | SKOV-3 cell line | Integrin -Linked Kinase (ILK) | Immunoblotting Zymography | ILK knockdown did not influence MMP-14 and MMP-2 expression and activity, but Smad-2 did via transforming growth factor-β1 (TGF-β1) |
Cowden Dahl [69] | 2007 | OVCA 433 cell line | Epidermal Growth Factor Receptor (EGFR) Ets transcription factor PEA3 | Q-PCR Zymography Matrigel invasion assay | Overexpression of the Ets transcription factor PEA3 induces EGFR-stimulated MMP-14 mRNA production |
Devine K [70] | 2008 | DOV13 and OVCA 429 cell lines | Sphingosine-1-Phosphate (S1P) G-protein Gi | Zymography Western blot | Low S1P increases MMP-2 activity Gi-dependent, high S1P decreases MMP-14 |
Agarwal A [40] | 2008 | SKOV-3 and OVCAR-3 in nude mouse model | PAR1-based pepcudins | RT-PCR cell migration assays in vivo mouse model | Cell-penetrating pepducins targeting this cascade resulted in inhibition of PAR1-dependent angiogenesis, ascites and metastasis |
Liao X [31] | 2009 | SKOV-3, OVCA 433 and OVCAR-3 cell lines | Hedgehog proteins Gli1 | RT-PCR Matrigel invasion assay | Increased Gli1 expression is correlated with increased MMP-14 expression and proliferation, cell mobility and invasiveness, inhibitors of HH pathway proteins result in a reversed effect |
Moss N [71] | 2009 | OVCA 433 cell line | Epidermal Growth Factor Receptor (EGFR) | Flow cytometry Western blot Three-dimensional collagen invasion assay | EGFR results in MMP-14 internalisation. Sustained surface MMP-14 causes enhanced migration and invasion |
Jiang L [72] | 2010 | SKOV-3 and OVCA 420 cell lines | Factor Binding to the Inducer of short transcripts of human immunodeficiency virus-1 (FBI-1) | Western blot Matrigel invasion assay | FBI-1 activates MMP-14 by binding to its promotor and thereby enhancing its expression and the invasive properties of the cell |
Poon S-L [73] | 2011 | OVCAR-3 and CaOV-3 cell lines | Gonadotropin-Releasing Hormone-II beta-catenin | Western blot Matrigel invasion assay | Gonadotropin-releasing hormone-II increases MMP-14 through the β-catenin signalling pathway and tincreases invasion |
Nakayama I [74] | 2013 | OVCAR-3, JHOC-5,-7,-8, JHOS-2,-3,-4 and JHOM-1 cell lines | miRNA-10b (non coding RNA) and HOXD10 (mRNA encoding a transcriptional repressor) | RT-PCR Western blot Matrigel invasion assay | Up-regulation of miR-10b results in loss of HOXD10, induces MMP-14 expression and invasion in ovarian cancer cell lines |
Yang Y [75] | 2016 | SKOV-3, A2780 and OMC685 cell line | Urothelial Carcinoma Associated 1 (UCA1) long noncoding RNA | Q-PCR Westen blot | UCA1 induces MMP-14 expression |
Semprucci E [76] | 2016 | SKOV-3, HEY, CaOV-3 cell lines | Endothelin A receptor beta-arrestin/PDZ-RhoGEF | Confocal scanning laser microscopy | Endothelin A receptor regulates the function of invadopodia, resulting in MMP-related invasion through the β-arrestin/PDZ-RhoGEF pathway |
Duan [77] | 2018 | SKOV-3, OVCAR-3 cell lines and mouse model | miR-122/P4HA1 | Migration and invasion assays, Western blot as well as tumour spread in nude mouse model | miR-122 inhibited migration, invasion, EMT, and metastasis in peritoneal cavity of ovarian cancer cells by targeting P4HA1 |