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Table 1 Molecules tested in ovarian cancer models with MMP-14 and MMP-2

From: The role of MMP-14 in ovarian cancer: a systematic review

First author Year Model Molecule(s) involved Technique Result
Kikkawa F [67] 2005 SKOV-3 cell line Dipepeptidyl peptidase IV (DPPIV) Immunoblotting DPPIV decreases invasion, increases TIMP and E-cadherin
Barbolina M [38] 2006 DOV13 cell line Epidermal Growth Factor Receptor (EGFR) Q-PCR Zymography Three-dimensional collagen invasion assay MMP-14 activation by collagen type I was investigated as measured by MMP-2 activation in zymography. This activation was induced only by EGR1 but not by EGR-2–4
Lin S-W [68] 2007 SKOV-3 cell line Integrin -Linked Kinase (ILK) Immunoblotting Zymography ILK knockdown did not influence MMP-14 and MMP-2 expression and activity, but Smad-2 did via transforming growth factor-β1 (TGF-β1)
Cowden Dahl [69] 2007 OVCA 433 cell line Epidermal Growth Factor Receptor (EGFR) Ets transcription factor PEA3 Q-PCR Zymography Matrigel invasion assay Overexpression of the Ets transcription factor PEA3 induces EGFR-stimulated MMP-14 mRNA production
Devine K [70] 2008 DOV13 and OVCA 429 cell lines Sphingosine-1-Phosphate (S1P) G-protein Gi Zymography Western blot Low S1P increases MMP-2 activity Gi-dependent, high S1P decreases MMP-14
Agarwal A [40] 2008 SKOV-3 and OVCAR-3 in nude mouse model PAR1-based pepcudins RT-PCR cell migration assays in vivo mouse model Cell-penetrating pepducins targeting this cascade resulted in inhibition of PAR1-dependent angiogenesis, ascites and metastasis
Liao X [31] 2009 SKOV-3, OVCA 433 and OVCAR-3 cell lines Hedgehog proteins Gli1 RT-PCR Matrigel invasion assay Increased Gli1 expression is correlated with increased MMP-14 expression and proliferation, cell mobility and invasiveness, inhibitors of HH pathway proteins result in a reversed effect
Moss N [71] 2009 OVCA 433 cell line Epidermal Growth Factor Receptor (EGFR) Flow cytometry Western blot Three-dimensional collagen invasion assay EGFR results in MMP-14 internalisation. Sustained surface MMP-14 causes enhanced migration and invasion
Jiang L [72] 2010 SKOV-3 and OVCA 420 cell lines Factor Binding to the Inducer of short transcripts of human immunodeficiency virus-1 (FBI-1) Western blot Matrigel invasion assay FBI-1 activates MMP-14 by binding to its promotor and thereby enhancing its expression and the invasive properties of the cell
Poon S-L [73] 2011 OVCAR-3 and CaOV-3 cell lines Gonadotropin-Releasing Hormone-II beta-catenin Western blot Matrigel invasion assay Gonadotropin-releasing hormone-II increases MMP-14 through the β-catenin signalling pathway and tincreases invasion
Nakayama I [74] 2013 OVCAR-3, JHOC-5,-7,-8, JHOS-2,-3,-4 and JHOM-1 cell lines miRNA-10b (non coding RNA) and HOXD10 (mRNA encoding a transcriptional repressor) RT-PCR Western blot Matrigel invasion assay Up-regulation of miR-10b results in loss of HOXD10, induces MMP-14 expression and invasion in ovarian cancer cell lines
Yang Y [75] 2016 SKOV-3, A2780 and OMC685 cell line Urothelial Carcinoma Associated 1 (UCA1) long noncoding RNA Q-PCR Westen blot UCA1 induces MMP-14 expression
Semprucci E [76] 2016 SKOV-3, HEY, CaOV-3 cell lines Endothelin A receptor beta-arrestin/PDZ-RhoGEF Confocal scanning laser microscopy Endothelin A receptor regulates the function of invadopodia, resulting in MMP-related invasion through the β-arrestin/PDZ-RhoGEF pathway
Duan [77] 2018 SKOV-3, OVCAR-3 cell lines and mouse model miR-122/P4HA1 Migration and invasion assays, Western blot as well as tumour spread in nude mouse model miR-122 inhibited migration, invasion, EMT, and metastasis in peritoneal cavity of ovarian cancer cells by targeting P4HA1