Table 3 Primer sequences used for the analysis of 13 ovarian ageing-related genes
From: Effects and mechanisms of mUCMSCs on ovarian structure and function in naturally ageing C57 mice
Gene ID Gene name | Primer sequence (5'āāā3') | Product length (bp) | Annealing temperature (Ā°C) |
|---|---|---|---|
NM_001127233.1 (P53) | F: ATGAACCGCCGACCTATCCT | 264 | 60.76 59.13 |
R: GCGGATCTTGAGGGTGAAATAC | |||
NM_013671.3 (SOD2) | F: TCCCAGACCTGCCTTACGACT | 244 | 61.1 60.4 |
R: CCCTTAGGGCTCAGGTTTGTC | |||
NM_009810 (CASP3) | F: GTCTGACTGGAAAGCCGAAAC | 205 | 58.9 59.5 |
R: GACTGGATGAACCACGACCC | |||
NM_007527 (Bax) | F: GCCTTTTTGCTACAGGGTTTCAT | 151 | 61.9 59.9 |
R: TATTGCTGTCCAGTTCATCTCCA | |||
NM_009741.5 (Bcl2) | F: GCTACCGTCGTGACTTCGCA | 270 | 61.9 61.6 |
R: CATCCCAGCCTCCGTTATCC | |||
NM_019584.3 (Beclin1) | F: GCAGCAGTTCAAAGAAGAGGTG R: TTTTGATGGAATAGGAGCCGC | 122 | 60.3 58.7 |
NM_026160.4 (LC3b) | F: CGTCCTGGACAAGACCAAGTTC R: GCAAGCGCCGTCTGATTATC | 80 | 61.12 59.77 |
NM_001159589.1 (sirt1) | F: CAGCATCTTGCCTGATTTGTAA R: TGGGGTATAGAACTTGGAATTAGTG | 268 | 57.29 58.1 |
NM_011034.4 (PRDX I) | F: CCACGGAGATCATTGCTTTCA | 139 | 58.64 58.55 |
R: GGCCCAATCCTCCTTCTTTC | |||
NM_001313711.1 (PRDX IV) | F: TGTTGCGGACCGAATCTCTG R: CAATAAGGTGCTGGCTTGGAGA | 178 | 60.39 60.62 |
NM_001358444.1 (PRDX V) | F: AAGAAGCAGGTTGGGAGTGTG R: AACAGCCAGGTGTAAATGCCC | 201 | 60.48 61.17 |
NM_001303408.1 (PRDX VI) | F: CACCACAGGAACTGGCAGAC R: TGGTGGCAGGGTAGAGGAAG | 317 | 61.47 60.41 |
NM_007393.3 (Ī²-actin) | F: GTGACGTGACATCCGAAAGA R: GTAACAGTCCGCCTAGAAGCAC | 287 | 58.7 61.0 |