Fig. 4

Hsa_circ_0004712 knockdown blocked OC development in vitro by upregulating miR-331-3p. OVCAR-3 and SKOV-3 cells were transfected with si-hsa_circ_0004712, si-NC, si-hsa_circ_0004712 + anti-miR-331-3p or si-hsa_circ_0004712 + anti-NC. A The expression of miR-331-3p in these transfected cells. B Cell proliferation detected by CCK-8 assay. C Colony formation ability detected by colony formation assay. D Cell apoptosis detected by flow cytometry. E Cell invasion detected by transwell assay. F Cell migration detected by wound healing assay. G and H The expression of C-caspase 3, MMP2 and MMP9 detected by western blot. I and J The activities of MMP2 and MMP9 were determined by gelatin zymography analysis. *P < 0.05