Fig. 1

Establishment and characterization of taxol and carboplatin resistant SOC cell lines. A The growth of SOC cells (HO8910, HO8910-TAX, HO8910-CBP, HO8910-TAX/CBP, SKOV3, SKOV3-TAX, SKOV3-CBP and SKOV3-TAX/CBP) in the presence of treatment with 50 μg/ml taxol or (and) 100 μg/ml carboplatin for 5 days. B Cell survival rate was evaluated by CCK-8 assay in the presence of taxol or (and) carboplatin. Parental and taxol and carboplatin resistant cells were treated with different drug concentrations, then the 50% inhibitory concentration (IC50) was calculated. C qRT-PCR (a) and Western blot(b) analysis of the expression of MDR1, ERCC1 and Bcl2 in SOC cell lines. The mRNA and protein levels of analyzed genes were normalized by those of β-actin in the experiment. D The cell lines were grown in the absence of drug treatment, and the cell proliferation of SOC cells was tested by CCK-8 assay. E The cell lines were grown in the absence of drug treatment, and the colony formation of SOC cells was assessed. F Cell cycle analysis by flow cytometry on TAX or (and) CBP-resistant and parental cells, when they are grown in the absence of drug treatment. *P < 0.05, **P < 0.01, ***P < 0.001. Data in this figure are presented as the mean ± SD of three independent experiments