Fig. 3

AFF4 is a direct target of miR-425-5p in ovarian cancer cells. A To predict potential targets of miR-425-5p using multiple miRNA-target prediction tools, the miR-425-5p–target interaction network was visualized using Cytoscape 3.7.1. BAFF4 expression in ovarian cancer cells (A2780, SKOV3, and ES-2) and IOSE-80 cells was detected by real-time quantitative polymerase chain reaction (RT-qPCR). C Schematic diagram showing the binding sites of miR-425-5p and AFF4 3’UTR and the mutation site of AFF4 3’UTR. D Relative luciferase activities were detected by dual-luciferase reporter assays in SKOV-3 and ES-2 cells after co-transfection of miR-425-5p mimics (miR mimics) or negative control mimics (NC mimics) with wild-type-AFF4-luc (WT) and mutant-AFF4-luc (MUT). EMiR-425-5p expression in SKOV-3 and ES-2 cells was determined by RT-qPCR, after transient transfection of miR-425-5p inhibitor (miR inhibitor) and negative control inhibitor (NC inhibitor). F The mRNA and protein expressions of AFF4 in SKOV-3 and ES-2 cells were determined by RT-qPCR and western blot analysis, after transient transfection of miR-425-5p inhibitor (miR inhibitor) and NC inhibitor. miR inhibitor, miR-425-5p inhibitor; NC inhibitor, corresponding negative control of the miR-425-5p inhibitor; miR mimics, miR-425-5p mimics; NC mimics, negative control of miR-425-5p mimics. ^N.S.p > 0.05, ^#p < 0.05, ^##p < 0.01, ^###p < 0.001