Fig. 3

Ghrelin enhanced the suppression of tumor growth by cisplatin in vitro. A-B Representative of MTT assay (A) or BrdU incorporation assay (B) of HO-8910 PM under ghrelin (10 nM) treatment in the presence or absence of cisplatin (1 μg/ml). C-F Alterations of the apoptosis (C-D) and the cell cycle distribution (E-F) measured in HO-8910 PM after treatment with ghrelin, cisplatin and the combination of ghrelin with cisplatin. G-H Western blotting analysis of the expression of p-CDK1, cyclin B1, and p21 in HO-8910 PM after treatment with ghrelin, cisplatin and the combination of ghrelin with cisplatin for 12 h. β-actin was used as a loading control. Data are shown as the mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001