Fig. 2

Knockdown of MNX1-AS1 inhibits OC cell proliferation, blocks cell cycle and promotes cell apoptosis. MNX1-AS1 shRNA lentivirus and NC shRNA lentivirus were transfected into SKOV-3 and OVCAR-3 cells, respectively. A RT-qPCR detected the expression of MNX1-AS1. B Cell viability was detected by CCK8 assay. C Cell proliferation was detected by colony formation assay. D Cell cycle was detected by flow cytometry. E Western blot was used to detect the expression of cell cycle-related genes. F Flow cytometry detected cell apoptosis. G The protein levels of apoptosis-related genes were detected by western blot. ^*P<0.05, ^**P<0.01, the difference comparison was compared with sh-NC transfected cells. Error bars were represented the mean ± SD in three independent repetitions