Fig. 3

Melatonin increases mitochondrial membrane potential and promotes p-Akt expression in the DHT-treated KGN cells. A-B Level of mitochondria membrane potential in the DHT group was lower than the control group, and melatonin treatment attenuated the decrease. Ratio of JC-1 aggregate/JC-1 monomer was measured for mitochondrial membrane potential. (n = 3 in each group) C-D Level of mPTP AOD (average optical density) was also significantly decreased in the KGN cells and increased after treated with melatonin. (n = 3 in each group) E-H The level of p-Akt and SIRT1 protein expression was decreased when treated with DHT, and melatonin treatment attenuated this change. (n = 3 in each group) NS, not significant, P > 0.05, *P < 0.05, **P < 0.01