Fig. 5

Effects of SIRT1 on Akt activation in KGN cells. A-C DHT treatment decreased p-PDK1 expression, and melatonin attenuated this change. While total PDK1 expression showed no significant difference. (n = 3 in each group) D SIRT1 was knocked down by SIRT1 siRNA. (n = 3 in each group) E-F Melatonin treatment failed to increase the p-PDK1 expression and activated Akt level in the SIRT1 siRNA transfected KGN cells. (n = 3 in each group) G-J Melatonin receptors MTNR1A and MTNR1B expression showed no significant difference in the KGN cells by fluorescent microscopy. (n = 3 in each group) *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001