Fig. 4

The effects of CXCL14 knockdown or overexpression on Wnt/β-catenin signaling-related markers. A Representative images of immunohistochemical staining of β-catenin in surgical specimens from OC tissues and non-tumor ovarian tissues (original magnification, × 100). B Correlation study of CXCL14 and β-catenin in OC tissues. Pearson correlation coefficient were used for statistical analysis of the correlation between CXCL14 and β-catenin. C Western blotting shows that overexpressing CXCL14 in OC cells increased the nuclear accumulation of β-catenin, respectively. ^**P < 0.01 compared with vector. D-E Following CXCL14 shRNA or overexpression treatment, qRT-PCR detection of the mRNA levels (cyclin D1, MMP-7, Axin2) in OC cells. ^**P < 0.01. F-G Following CXCL14 shRNA or overexpression treatment, western blotting detection of the protein expressions of cyclin D1, MMP-7 and Axin2 in OC cells. ^**P < 0.01 compared with sh-NC or vector