Fig. 5

miR-3187-3p directly targets ERBB4. A, a Venn diagram for the intersections of the candidate target mRNAs of miR-3187-3p from five bioinformatic systems; B, a KEGG pathway enrichment analysis based on the candidate mRNAs; C, expression of ERBB4 in OC predicted on the GEPIA database; D, mRNA expression of ERBB4 in the collected tissue samples detected by RT-qPCR; E, a negative correlation between the expression of miR-3187-3p and ERBB4 mRNA in OC tissues; F, mRNA expression of ERBB4 in ES-2 and SK-OV-3 cells after oe-circ/sh-circ/miR-mimic/miR-inhibitor transfection examined by RT-qPCR; G, putative binding sequence between miR-3187-3p and ERBB4 3’UTR and the MT sequence for luciferase assay; H, luciferase activity of circ-WT and circ-MT in 293 T cells examined by the luciferase reporter gene assay. Data were exhibited as mean ± SD from three repetitions. Differences were analyzed by paired t test (D), one-way ANOVA (F) or two-way ANOVA (H); *p < 0.05 vs. adjacent; #p < 0.05 vs. oe-NC; &p < 0.05 vs. oe-circ +NC mimic; @p < 0.05 vs. sh-NC; ^p < 0.05 vs. sh-circ + NC inhibitor; %p < 0.05 vs. NC mimic; In panel E, the correlation between miR-3187-3p and ERBB4 was validated using Pearson’s correlation analysis, r = − 0.702, p < 0.001