Table 1 Primer sequences used for real time PCR analysis
Gene (Mus musculus) | Forward primer(5′-3′) | Reverse primer(5′-3′) | Annealing temperature(°C) | Product size (bp) |
|---|---|---|---|---|
Gdf9 | AAAGAAGACTGGCACGAGGA | CAAAGCAGCAAACCCCCAA | 59 | 92 |
Bmp15 | TGGGTTCCTAAATGCCGGAC | TGCCTTTTTGCTGCTGACAC | 60 | 181 |
Gpx1 | TGCAATCAGTTCGGACACCA | TCTCACCATTCACTTCGCACT | 59 | 130 |
Sod1 | AGCATTCCATCATTGGCCGT | TCCCAGCATTTCCAGTCTTTGT | 60 | 99 |
Bcl2 | AATTGTAATTCATCTGCCGCCG | GTGGAGGAAAAATCAGGAGGGT | 60 | 150 |
Bax | ACATGGCAGACAGTGACCAT | AAGACACAGTCCAAGGCAGT | 59 | 103 |
β-actin | AAGTACTCTGTGTGGATCGGTG | GGTGTAAAACGCAGCTCAGTAA | 59 | 153 |