Fig. 1

Composite image showing darkfield maximum intensity projection and 4 channel fluorescence of different cell lineages. Legend: Monocytes exhibit high PNP-binding, the B cell medium PNP-binding, and other lineages little or no binding. Fluorophore conjugated anti-CD 45 antibodies were applied as a pan-leukocyte marker visible as green in channel 0. Monocytes were identified with fluorophore conjugated anti-CD14 seen as green on channel 1. B cells were identified using fluorophore conjugated anti-CD 19, visible as blue in channel 0. NK cells with fluorophore conjugated anti-CD56, shows as green at channel 0 and orange in channel 1 and channel 2. T cells were identified using fluorophore conjugated anti-CD3, which appear as red in channel 3. Finally, NK-T cells were identified as green in channel 0, orange in channel 1 and 2 and red in channel 3