Fig. 4

MiR-4324 targeting FEN1 attenuated the progression of ovarian cancer. A Measurement of FEN1 expression in CaOV3 and OVCAR3 cells transfected with NC, Si- FEN1, and Si- FEN1+ inhibitor by RT-qPCR. B Measurement of FEN1 protein expression in CaOV3 and OVCAR3 cells transfected with NC, Si- FEN1, and Si- FEN1+ inhibitor by western blot. C Cell viability was detected in CaOV3 and OVCAR3 cells transfected with NC, Si- FEN1, and Si- FEN1+ inhibitor by CCK-8 assay. D Cell proliferation was detected in CaOV3 and OVCAR3 cells transfected with NC, Si- FEN1, and Si- FEN1+ inhibitor by BrdU assay. E Colony formation number was detected in CaOV3 and OVCAR3 cells transfected with NC, Si- FEN1, and Si- FEN1+ inhibitor by colony formation assay. F Cell apoptosis level was determined in CaOV3 and OVCAR3 cells transfected with NC, Si- FEN1, and Si- FEN1+ inhibitor by Caspase 3 kit. G Bax and Bcl-2 protein levels were detected in CaOV3 and OVCAR3 cells transfected with NC, Si- FEN1, and Si- FEN1+ inhibitor by western blot assay. H Cell adhesion was detected in CaOV3 and OVCAR3 cells transfected with NC, Si- FEN1, and Si- FEN1+ inhibitor. I Cell migration rate was detected in CaOV3 and OVCAR3 cells transfected with NC, Si- FEN1, and Si- FEN1+ inhibitor by wound healing assay. *, P < 0.05; **, P < 0.001. NC, negative control; Si- FEN1, SiRNA-FEN1; inhibitor, miR-4324 inhibitor; Si- FEN1+ inhibitor, SiRNA-FEN1 + miR-4324 inhibitor