Fig. 2
From: Mutation analysis of the TUBB8 gene in primary infertile women with oocyte maturation arrest
Phenotypes of oocytes from patients with mutations of TUBB8. a The morphologies of the MI or MII oocytes examined by light microscopy. The arrow represents the polar body. b The protein 3D structure of the disease associated variants rendered by SWISS-MODEL program. The variation of p.A313V was polar, neutral amino acid in the β-fold region, while p.C239W occurred in the α-helix region. The variation of p.R251Q changed from negatively charged amino acid to positively charged amino acid, which affected the formation of hydrogen bond in the secondary structure of protein and may affect the structural stability of protein. p.P358L was nonpolar amino acid. The variation of p.G96R changed from polar, neutral to positively charged amino acid