Fig. 3
From: Mutation analysis of the TUBB8 gene in primary infertile women with oocyte maturation arrest
TUBB8 expression analysis in cultured cells. a-b The expression level of WT, p.A313V, p.G96R, p.C239W, and p.R251Q TUBB8 protein in Hela cells detected by Western Blot. c-d Microtubule morphology and phenotypes of Hela cells transfected with constructs engineered to express C-terminally FLAG-tagged TUBB8 (WT and mutants) and examined by immunofluorescence microscopy. The cells were stained with DAPI to visualize nucleus (blue), FLAG to visualize transgene (green) and α-tubulin to visualize endogenous microtubule network (red)