Fig. 2

Effects of circHRHOBTB3 on proliferation, invasion, cell cycle distribution, and apoptosis in OC cells. The transfection groups were respectively: si-circ, si-NC, circ, circ-NC groups. A The growth curves of cells were analyzed using CCK8 assay (Student’s t-test). B The cell cycle distribution was examined by flow cytometry (Student’s t-test). C Invasion abilities of EOC cells were evaluated by transwell matrigel invasion assay. (Student’s t-test), D. Cell apoptosis was examined by Flow cytometry. Quadrant 1 (Q1) represented a flowchart of dead cells; Quadrant 2 (Q2) represented inactive apoptotic cells; Quadrant 3 (Q3) represented viable apoptotic cells; Quadrant 4 (Q4) represented viable non-apoptotic cells. (Student’s t-test)