Fig. 2
From: miR-600 promotes ovarian cancer cells stemness, proliferation and metastasis via targeting KLF9
miR-600 knockdown inhibits ovarian cancer stem cells expansion. A. The knockdown effect of miR-600 in HO8910 and A2780 cells was checked by real-time PCR analysis. B. Representative images of ovarian cancer spheroids generated from miR-600 knockdown ovarian cancer cells and control cells. The number of spheroids was counted and compared. C. The expression of SOX2, OCT4, c-Myc and Nanog in miR-600 knockdown ovarian cancer cells and control cells was determined by real-time PCR assay. D. The frequency of ovarian cancer stem cells in miR-600 knockdown ovarian cancer cells and control cells was compared by in vitro limiting dilution assay. E. miR-600 knockdown ovarian cancer cells and control cells were inoculated into NOD-SCID mice subcutaneously, and the tumorigenicity was evaluated two months post inoculation. The frequency of CSCs was calculated