Fig. 4
From: miR-600 promotes ovarian cancer cells stemness, proliferation and metastasis via targeting KLF9
KLF9 is the directly target of miR-600 in ovarian cancer cells. A. A potential target site for miR-600 in the 3′-UTR of human KLF9 mRNA, as predicted by the program Targetscan and miRBase. To disrupt the interaction between miR-600 and KLF9 mRNA, the target site was mutated. B. Luciferase reporter assays of miR-600 knockdown ovarian cancer cells and control cells transfected with wild-type or mutant KLF9 3′-UTR constructs. C. Real-time PCR analysis KLF9 mRNA expression in miR-600 knockdown ovarian cancer cells and control cells. D. Western blot analysis of KLF9 protein expression in miR-600 knockdown ovarian cancer cells and control cells. GAPDH was used as the loading control. E. Significant correlation was observed between miR-600 and KLF9 expression in human ovarian cancer tissues (n = 34)