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Table 1 Ovarian reserve of eggs determined by the number of ovarian follicles at different stages of development

From: Super-critical fluid extract of Bryonopsis laciniosa (Shivlingi) seeds restores fertility in zebrafish models through revival of cytological and anatomical features

 

Study Groups

Control

Fertility impaired model

Letrozole (0.036 µg/kg)

SLSO (µg/kg)

0.25

0.64

1.28

6.4

12.8

128

Follicle count—Ovarian Reserve of Eggs

Primary growth (PG)

114 ± 8.2

97 ± 6.8####

137 ± 7****

99 ± 9.2

113 ± 8.5****

320 ± 6.9****

352 ± 5.9****

458 ± 7.9****

389 ± 9.8****

Pre-Vitellogenic (PV)

296 ± 8.1

79 ± 8.9####

256 ± 7.7****

83 ± 8.5

94 ± 6.3****

490 ± 6.9****

291 ± 7.9****

125 ± 8.4****

131 ± 6.6****

Early Vitellogenic (EV)

311 ± 8.3

16 ± 6.8####

140 ± 7.4****

27 ± 8.5****

31 ± 7.8****

34 ± 7.5****

39 ± 7.4****

208 ± 8.4****

264 ± 7.5****

Mid-Vitellogenic (MV)

109 ± 7.8

9 ± 7.6####

96 ± 8.4****

10 ± 5.8

20 ± 6.3****

31 ± 8.0****

28 ± 7.2****

49 ± 7.0****

64 ± 9.1****

Final growth (FG)

483 ± 7.6

12 ± 6####

356 ± 7.6****

31 ± 7.1****

91 ± 8.0****

119 ± 7.7****

196 ± 8.4****

321 ± 8.6****

401 ± 7.8****

Total

1313 ± 16.3

213 ± 15.3####

985 ± 18.0****

250 ± 17.0****

348 ± 18.4****

993 ± 15.6****

906 ± 18.4****

1161 ± 17.6****

1249 ± 19.0****

  1. Data represented as mean ± SD (n = 24). Statistical significance was arrived at using ANOVA followed by Tukey’s multiple comparison test
  2. #### represents p < 0.0001 compared to normal control, **** represents p < 0.0001 compared to the fertility impaired group