Fig. 2

MAEL influences the invasion and metastasis of human EOC cells. A Western blot analysis confirmed that MAEL was effectively knocked down by transfection of MAEL-shRNAs into ES2 and SKOV3 cells. B Western blot analysis confirmed that MAEL was substantially increased in stable MAEL-transfected OVCAR3 and HO8910 cells.C Wound-healing showing the migration and transwell invasion assay showing the invasion of MAEL-shRNAs-transfected ES2 and SKOV3 cells by comparison with the corresponding control cells. Data are presented as the mean ± SD of three independent experiments (***p < 0.001). D Wound-healing showing the migration and transwell invasion assay showing the invasion of MAEL-transfected OVCAR3 and HO8910 cells by comparison with the corresponding control cells. Data are presented as the mean ± SD of three independent experiments (***p < 0.001). E Representative lungs with metastatic nodules (tagged by arrow heads) and lung metastatic tumors stained with hematoxylin and eosin (H&E). Number of lung metastatic nodules four weeks after injection of ES2-shMAEL-1 and ES2-shControl stable clones (n = 8, p < 0.05)