Fig. 2

YY1 promotes Kcnq1ot1 transcription and elevates Kcnq1ot1 expression to promote OSCC progression. A RT-qPCR was performed to detect YY1 expression in OSCC tissues and normal tissues, n = 98; B ChIP assay was implemented to verify the binding relation between Kcnq1ot1 and YY1; C RT-qPCR was utilized to determine YY1 and Kcnq1ot1 expression after down-regulating or up-regulating YY1; D Pearson test was carried out to analyze the correlation between Kcnq1ot1 and YY1 expression in OSCC tissues, n = 98; E. RT-qPCR and Western blot assay were employed to test YY1 expression in cells in response to cotransfection of pc-YY1 and si-Kcnq1ot1; F RT-qPCR for detecting Kcnq1ot1 expression in cells in response to cotransfection of pc-YY1 and si-Kcnq1ot1; G-H MTT assay and colony formation assay were utilized to determine cell proliferation in cells in response to cotransfection of pc-YY1 and si-Kcnq1ot1; I Flow cytometry for measuring cell apoptosis in cells in response to cotransfection of pc-YY1 and si-Kcnq1ot1; J-K Transwell assay was carried out to detect cell migration and invasion in cells in response to cotransfection of pc-YY1 and si-Kcnq1ot1; * P < 0.05 compared with the Vector group; $ P < 0.05 compared with pcYY1 group; Measurement data were expressed as mean ± standard deviation