Fig. 2

Presence of living endometrial cells in cyst fluid and drainage fluid. A The white arrow shows the endometrial fragments filtered out from cyst fluid. B H&E staining of endometrial fragments; × 200; bar = 50 μm. C IHC staining of cytokeratin 19 in endometrial fragments; × 200; bar = 50 μm. D IHC staining of CD10 in endometrial fragments; × 200; bar = 50 μm. E Negative control for immunohistochemistry staining in endometrial fragments; × 200; bar = 50 μm. F Morphology and identification of primary cultured cells from endometrial fragments. Bright field, × 100, bar = 100 μm; IF field; × 200; bar = 50 μm. Stromal cells (vimentin in green) and epithelial cells (cytokeratin19 in red). G Representative images of tissue in the drainage bag from endometriosis and non-endometriosis patients. H Representative images of H&E staining in tissue from the drainage fluid of endometriosis; × 400; bar = 20 μm. I Representative images of IHC staining of cytokeratin 19 in tissue from the drainage fluid of endometriosis (Negative control was in the box in the upper left corner); × 400; bar = 20 μm