Fig. 5
From: Neuregulin-1 signaling regulates cytokines and chemokines expression and secretion in granulosa cell
Representative images of immunocolocalization of neuregulin-1 (NRG1) and epidermal growth factor receptor 3 (ErbB3) in the rat ovarian follicles. Ovaries were collected from pregnant mare serum gonadotropin (PMSG)-treated and the estrus stage rats for immunohistochemistry (IHC) (n = 3 rats per group). Immunolocalization of endogenous NRG1 and ErbB3 were with Alexa Fluor 594-labeled (red) and Alexa Fluor 488-labeled (green) secondary antibodies respectively. The nucleus was counter stained with 4′,6′-diamidino-2-phenylindole (blue). A Representative photomicrographs of transverse- and sagittal-sections of antral-follicle in PMSG-treated ovary. B Transverse section of pre-ovulatory follicle (POF) during estrus stage showing cumulus-oocyte-complex (COC). A-antrum, GC-granulosa cells, C-cumulus, M-mural, O-oocyte, TIC-theca-interstitial cells