Fig. 3

Circ-BNC2 acts as a sponge of miR-223-3p. A The subcellular fractionation assay was used to detect the expression of circ-BNC2 in the nucleus and cytoplasm of SKOV3 and HO-8910 cells. B Bioinformatics tools was used to predict the binding site of circ-BNC2 and miR-223-3p. C Dual-luciferase reporter gene assay was used to validate the specific binding between circ-BNC2 and miR-223-3p in SKOV3 and HO-8910 cells. D RNA pull-down assay was carried out to verify the interaction between miR-223-3p and circ-BNC2 in SKOV3 and HO-8910 cells. E qRT-PCR was used to detect the expression of miR-223-3p in OC tissues and normal tissues adjacent to tumors. n = 40. F Pearson’s correlation coefficient analysis was performed to analyze the correlation between circ-BNC2 and miR-223-3p expression in OC tissues. G qRT-PCR was used to detect the expression of miR-223-3p in OC cells after silencing or overexpressing circ-BNC2. ***P < 0.001