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Fig. 4 | Journal of Ovarian Research

Fig. 4

From: Hexokinase 2 promoted cell motility and proliferation by activating Akt1/p-Akt1 in human ovarian cancer cells

Fig. 4

HK2 elevated Akt1 and p-Akt1 expression in human ovarian cancer cells. The protein level of Akt1 and p-Akt1 was detected by western blot and the quantitative analysis is shown: (A) SKOV3-GFP and SKOV3-HK2; (B) A2780-shCtr and A2780-shHK2. C The protein level of Akt1, p-Akt1, Fibronectin, MMP9, p21 and p27 was detected by western blot in MK2206 treated SKOV3-HK2 cells and the quantitative analysis is shown. D The protein level of Akt1, p-Akt1, Fibronectin, MMP9, p21 and p27 was detected by western blot in A2780-shHK2 cells that transiently transfected with an Akt1 recombinant plasmid, and quantitative analysis is shown. E The expression of HK2, p-Akt1, MMP9 and FN1 was detected in serial sections of human ovarian cancer tissues by using immunocytochemistry analysis (scale bar, 50 and 10 μm). The correlation between HK2 and p-Akt1(F), Fibronectin (G), MMP9 (H), in human ovarian cancer tissues was confirmed by using Pearson correlation analysis, n = 25

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