Fig. 1

Genetically encoded, fluorescent biosensors allow tracking of dynamic changes in OC metabolism. A Representative fluorescence micrographs verifying expression of HyPer, iNap, Perceval, and Peredox biosensors. Quantification of HyPer (B), iNap (C), Perceval (D), and Peredox (E) signals was performed on transfected SKOV3 cells grown in regular media vs. omental conditioned media (OCM) for 3, 12, 24, and 48 hours. Signals were normalized to regular media controls (n = 3, technical replicates). Statistical significance was calculated using an unpaired, two-tailed Students t-test (*p < 0.05, **p < 0.01)