Fig. 3

OC cell responses to carboplatin-induced oxidative stress are correlated with carboplatin resistance. A Metabolic changes in ATP/ADP, NADH/NAD+, oxidative stress, and NADPH/NADP+ induced by treatment with carboplatin varied across cell lines derived from primary tumor vs. ascites. Color bars represent the degree of change (green = greater change, red = minimal change from baseline). B HyPer-expressing cell lines were treated with 100 μM carboplatin and HyPer signal was measured at 24 and 48 hours. C HyPer responses in carboplatin-treated cells were compared to untreated cells normalized to 1 (n = 3 per condition). D Normalized HyPer responses for each cell line were plotted against reported carboplatin IC50s, and a simple linear regression demonstrated a positive correlation (r = 0.53, p = 0.041)