Fig. 3

Secondary follicle granulosa cells have evidence of DNA damage/repair, increased growth, and atresia at 14D. Graph statistics include t tests, and distributions with KS test. The distribution and median of staining for (A, B) P-eIF2ɑ in secondary follicle granulosa cells (p = 0.01, p = 0.0036). C, D The number of secondary follicle granulosa cells positively stained per specimen for ɣ-H2AX (p = 0.03) at 14D. Yellow arrows denote positively stained granulosa cells, green stars show primordial oocytes, which only stained positive in WT samples (D). Global staining for (E) Ki67 at 14D in GalTKO ovaries compared to WT. Secondary follicle granulosa cells positively stained for (F,G) CCASP3 (p = 0.01). White dashed circles show outline of follicles