Fig. 1

Plumbagin attenuates PCOS in mice by preventing granulosa cell death. A H&E staining for the ovary structure. The study includes control mice, PCOS mice and PCOS mice treated with Plumbagin. B, C GCs were purified from the ovary of three groups of mice, cell survival was determined by three different approaches (n = 5). B. LDH release by ovarian GCs in the culture supernatant. C Relative cell viability index determined by the fluorescent intensity of alamarBlue™ Cell Viability Reagent staining. D-I Plasma was isolated from mice in three study groups, reproduction hormone levels were measured by ELISA (n = 6). D. Follicle-Stimulating Hormone (FSH). E Luteinizing Hormone (LH). F Testosterone. G Estradiol(E2). H Anti-Müllerian Hormone (AMH). I Progesterone (P4). Individual data points are displayed. Data are mean ± SEM. Unpaired one-way ANOVA was used to analyze the difference. *P < 0.05; **P < 0.01; ***P < 0.001