Fig. 3

Caspase 1 inhibitor Vx-765 rescues the PCOS pathology by preventing the pyroptosis of GCs. PCOS mice were treated with Caspase 1 inhibitor Vx-765. A H&E staining for the ovary structure. B The LDH released by GC cells. N = 4. C GC viability was measured by alamarBlue™ Cell Viability Reagent staining. N = 6. D Immunoblot to quality active fragment Caspase-1-p10 and GSDMD-N. E Relative protein expression of Caspase-1-p10 and GSDMD-N normalized with b-actin. N = 3. G IL-1β concentration in the culture supernatant was measured by ELISA after 24 hours. H The amount of IL-18 released to the culture medium after 24 hours. N = 5. Individual data points are displayed. Data are mean ± SEM. Unpaired student t-test were used to analysis the difference. *P < 0.05; **P < 0.01; ***P < 0.001