Fig. 4

Plumbagin protects against the pyroptotic death of Granulosa cells. GC cell line HGL5 was cultured in presence of pyroptosis inducer nigericin and plumbagin. A Cell survival was determined by LIVE/DEAD™ Cell Imaging. B The production of LDH by dead cells was quantified. N = 4. C The cell viability of HGL5 was measured by alamarBlue staining. N = 4. D Activation of Caspase-1 indicated by staining with FLICA probe (Representative graph). E Activation of Caspase-1 indicated by staining with FLICA probe (statistic). N = 4. F IL-1β concentration in the culture supernatant was measured by ELISA. N = 4. G IL-18 release to the culture medium was quantified by ELISA. N = 4. Individual data points are displayed. Data are mean ± SEM. Paired one-way ANOVA was used to analyze the difference. *P < 0.05; **P < 0.01; ***P < 0.001