Fig. 5

Plumbagin destabilizes the mRNA of the inflammasome component ASC. GCs were isolated from the ovary of mice. A The mRNA relative expression of inflammasome complex NLRP3,ASC and NEK7 were measured by RT-PCR. N = 4. B Protein accumulation of NLRP3,ASC and NEK7 were detected with immunoblot. C Relative protein expression of NLRP3,ASC and NEK7 normalized to b-actin. N = 3. D GCs were treated with transcription inhibitor actinomycin D, and the decay of ASC mRNA was measured every 2 hours for 8 hours. Individual data points are displayed in the bar graph. Data are mean ± SEM. one-way ANOVA was used to analyze the difference in A and C. Two-way ANOVA was used to analyze the difference in D. *P < 0.05; **P < 0.01; ***P < 0.001